Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 600 to 650 of human High Affinity Cationic Amino Acid Transporter 1 using the numbering given in entry NP_003036.1 (GeneID 6541).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CAT1 Antibody - BSA Free
Western Blot: CAT1 Antibody [NBP2-32271]
Western Blot: CAT1 Antibody [NBP2-32271] - Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CAT-1 antibody NBP2-32271 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: CAT1 Antibody [NBP2-32271]
Immunoprecipitation: CAT1 Antibody [NBP2-32271] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CAT-1 antibody NBP2-32271 used for IP at 6 ug per reaction. CAT-1 was also immunoprecipitated by rabbit anti-CAT-1 antibody BL16464. For blotting immunoprecipitated CAT-1, NBP2-32271 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for CAT1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CAT1/SLC7A1
Long Name
Cationic 1/Solute Carrier Family 7 Member 1
Alternate Names
ATRC1, HCAT1, REC1L, SLC7A1
Gene Symbol
SLC7A1
UniProt
Additional CAT1/SLC7A1 Products
Product Documents for CAT1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CAT1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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