Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Cathepsin A/Lysosomal Carboxypeptidase A Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse Cathepsin A/Lysosomal Carboxypeptidase A (Uniprot#: P16675-1; Met1-Tyr474)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images

Western Blot: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264]

Western Blot: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264]

Western Blot: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264] - Lane A: Hela Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:28 kDa
Immunoprecipitation: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264]

Immunoprecipitation: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264]

Immunoprecipitation: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98264] - Lane A:0.5 mg K562 Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-Mouse CTSA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CTSA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa

Applications

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Alternate Names

CTSA, Lysosomal Carboxypeptidase A

Gene Symbol

CTSA

Additional Cathepsin A/Lysosomal Carboxypeptidase A Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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