Cathepsin D Antibody (102) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90432

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 102 expressed in HEK293

Format

Azide and BSA Free
View all Cathepsin D Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Cathepsin D (Accession#: NP_034113.1; Met 1-Leu 410).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cathepsin D Antibody (102) - Azide and BSA Free

Western Blot: Cathepsin D Antibody (102) [NBP2-90432]

Western Blot: Cathepsin D Antibody (102) [NBP2-90432]

Western Blot: Cathepsin D Antibody (102) [NBP2-90432] - Anti-Cathepsin D rabbit monoclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: Cathepsin D Antibody (102) [NBP2-90432]

Immunoprecipitation: Cathepsin D Antibody (102) [NBP2-90432]

Immunoprecipitation: Cathepsin D Antibody (102) [NBP2-90432] - Mouse Cathepsin D was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 0.5 ul anti-Mouse Cathepsin D rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Cathepsin D rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa

Applications for Cathepsin D Antibody (102) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin D

Cathepsin D has been reported to be involved in malignant tumour progression and with prognosis of various human cancers.

Alternate Names

CTSD

Gene Symbol

CTSD

Additional Cathepsin D Products

Product Documents for Cathepsin D Antibody (102) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cathepsin D Antibody (102) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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