Caveolin-1 Antibody (2A7) - BSA Free

Novus Biologicals | Catalog # NBP3-26695

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2A7 expressed in HEK293

Format

BSA Free
View all Caveolin-1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Caveolin-1 [UniProt Q03135]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Caveolin-1 Antibody (2A7) - BSA Free

Caveolin-1 Antibody (2A7)

Western Blot: Caveolin-1 Antibody (2A7) [NBP3-26695] -

Western Blot: Caveolin-1 Antibody (2A7) [NBP3-26695] - Positive Western Blot detected in: THP-1 whole cell lysate.
All lanes: Caveolin-1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Caveolin-1 Antibody (2A7)

Flow Cytometry: Caveolin-1 Antibody (2A7) [NBP3-26695] -

Flow Cytometry: Caveolin-1 Antibody (2A7) [NBP3-26695] - Overlay Peak curve showing Hela cells stained with Caveolin-1 Antibody (2A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Caveolin-1 Antibody (2A7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Caveolin-1

Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.

Alternate Names

CAV1, Caveolin1, MSTP085, VIP21

Gene Symbol

CAV1

Additional Caveolin-1 Products

Product Documents for Caveolin-1 Antibody (2A7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Caveolin-1 Antibody (2A7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Caveolin-1 Antibody (2A7) - BSA Free

There are currently no reviews for this product. Be the first to review Caveolin-1 Antibody (2A7) - BSA Free and earn rewards!

Have you used Caveolin-1 Antibody (2A7) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies