CBL Antibody (6D12) - Azide and BSA Free

Novus Biologicals | Catalog # H00000867-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6D12

Format

Azide and BSA Free
View all CBL Primary Antibodies »

Product Specifications

Immunogen

CBL (NP_005179, 736 a.a. ~ 835 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSER

Specificity

CBL - Cas-Br-M (murine) ecotropic retroviral transforming sequence

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CBL Antibody (6D12) - Azide and BSA Free

Western Blot: CBL Antibody (6D12) [H00000867-M01]

Western Blot: CBL Antibody (6D12) [H00000867-M01]

Western Blot: CBL Antibody (6D12) [H00000867-M01] - Analysis of CBL expression in transfected 293T cell line by CBL monoclonal antibody (M01), clone 6D12.Lane 1: CBL transfected lysate (Predicted MW: 99.6 KDa).Lane 2: Non-transfected lysate.
Western Blot: CBL Antibody (6D12) [H00000867-M01]

Western Blot: CBL Antibody (6D12) [H00000867-M01]

Western Blot: CBL Antibody (6D12) [H00000867-M01] - CBL monoclonal antibody (M01), clone 6D12 Analysis of CBL expression in K-562.
Immunoprecipitation: CBL Antibody (6D12) [H00000867-M01]

Immunoprecipitation: CBL Antibody (6D12) [H00000867-M01]

Immunoprecipitation: CBL Antibody (6D12) [H00000867-M01] - Analysis of CBL transfected lysate using anti-CBL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CBL monoclonal antibody.
Sandwich ELISA: CBL Antibody (6D12) [H00000867-M01] - Detection limit for recombinant GST tagged CBL is 0.1 ng/ml as a capture antibody.

Applications for CBL Antibody (6D12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CBL

The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.

Long Name

Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL

Alternate Names

CBL2, RNF55

Entrez Gene IDs

867 (Human)

Gene Symbol

CBL

Additional CBL Products

Product Documents for CBL Antibody (6D12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CBL Antibody (6D12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail