CBL [p Tyr700] Antibody (M161)

Novus Biologicals | Catalog # NBP3-23248

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M161
View all CBL Primary Antibodies »

Product Specifications

Immunogen

Clone (M161) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 700 in human c-Cbl. This sequence is highly conserved in rat and mouse c-Cbl. [UniProt# P22681]

Modification

p Tyr700

Specificity

The antibody detects a 120 kDa protein in human Jurkat cells treated with pervanadate, but not in control cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for CBL [p Tyr700] Antibody (M161)

CBL [p Tyr700] Antibody (M161)

Western Blot: CBL [p Tyr700] Antibody (M161) [NBP3-23248] -

Western Blot: CBL [p Tyr700] Antibody (M161) [NBP3-23248] - Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-CBL (NBP3-23247; lanes 1 & 2) or anti-CBL (Tyr-700) (NBP3-23248; lanes 3 & 4).

Applications for CBL [p Tyr700] Antibody (M161)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CBL

c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).

Long Name

Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL

Alternate Names

CBL2, RNF55

Gene Symbol

CBL

Additional CBL Products

Product Documents for CBL [p Tyr700] Antibody (M161)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CBL [p Tyr700] Antibody (M161)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail