Cbl-c Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30088

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cbl-c Primary Antibodies »

Product Specifications

Immunogen

Between 300 and 350

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cbl-c Antibody - BSA Free

Cbl-c Antibody

Immunoprecipitation:Cbl-c AntibodyNBP3-30088] -

Immunoprecipitation:Cbl-c AntibodyNBP3-30088] -Detection of human CBLC by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBLC antibody NBP3-30088 (lot NBP3-30088-1) used for IP at 6 µg per reaction. CBLC was also immunoprecipitated by rabbit anti-CBLC antibody. For blotting immunoprecipitated CBLC, NBP3-30088 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Cbl-c Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cbl-c

Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57. Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cbl-c knock out mice show no obvious phenotype. Thus, the physiological role of Cbl-c is not known

Alternate Names

Cas-Br-M (murine) ecotropic retroviral transforming sequence c, Cas-Br-M (murine) ectropic retroviral transforming sequence c, CBL3, CBL-3, CBL-SL, RING finger protein 57, RNF57SH3-binding protein CBL-3, SH3-binding protein CBL-C, signal transduction protein CBL-C

Gene Symbol

CBLC

Additional Cbl-c Products

Product Documents for Cbl-c Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cbl-c Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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