Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cbl-B Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 450 and 500 of human Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b using the numbering given in entry NP_733762.2 (GeneID 868).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CBLB Antibody - BSA Free (NBP1-49919) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CBLB Antibody - BSA Free

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919]

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919]

Immunohistochemistry-Paraffin: CBLB Antibody [NBP1-49919] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Cbl-b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: CBLB Antibody [NBP1-49919]

Immunoprecipitation: CBLB Antibody [NBP1-49919]

Immunoprecipitation: CBLB Antibody [NBP1-49919] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cbl-b antibody NBP1-49919 used for IP at 3 ug/mg lysate. Cbl-b was also immunoprecipitated by rabbit anti-Cbl-b antibody NBP1-49920, which recognizes a downstream epitope. For blotting immunoprecipitated Cbl-b, NBP1-49920 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for CBLB Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cbl-B

CBLB is an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.

Long Name

Cbl Proto-Oncogene B

Alternate Names

CBLB, Nbla00127, RNF56

Gene Symbol

CBLB

Additional Cbl-B Products

Product Documents for CBLB Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CBLB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

Customer Reviews for CBLB Antibody - BSA Free

There are currently no reviews for this product. Be the first to review CBLB Antibody - BSA Free and earn rewards!

Have you used CBLB Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CBLB Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: One of our customers bought one vial of NBP1-49919, and he told me the website said it can be used in WB, but when he got the antibody, he find the website said as follows, the customer is a little confused about the WB (-). Does this mean that it can't be used in WB? Please give us some help and thanks.

    A: Regarding your customer's inquiry, our CBLB antibody (NBP1-49919) has been validated for IHC and it is not recommended to use this product for western blot. The negative mark (-) indicates this product should not be used for western blot. I am sorry if this has caused confusion for your customers.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies