Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Chromatin Immunoprecipitation Sequencing, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 482 and 532 of human chromobox homolog 2 using the numbering given in entry NP_005180.1 (GeneID 84733).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cbx2 Antibody - BSA Free
Chromatin Immunoprecipitation Sequencing: Cbx2 Antibody [NBP1-40342] - Localization of CBX2 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CBX2 antibody NBP1-40342 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CBX2 binding within a 1.5 Mb region of chromosome 1 as detected using anti-CBX2 antibody NBP1-40342. ChIP-seq validation performed by Diogenode, Denville, NJ.
![Immunoprecipitation: Cbx2 Antibody [NBP1-40342]](https://resources.rndsystems.com/images/products/Cbx2-Antibody-Immunoprecipitation-NBP1-40342-img0005.jpg "Immunoprecipitation: Cbx2 Antibody [NBP1-40342]")
Immunoprecipitation: Cbx2 Antibody [NBP1-40342]
Immunoprecipitation: Cbx2 Antibody [NBP1-40342] - Detection of human CBX2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBX2 antibody NBP1-40342 (lot NBP1-40342-3) used for IP at 6 ug per reaction. CBX2 was also immunoprecipitated by previous lots of this antibody (lots NBP1-40342-1 and NBP1-40342-2). For blotting immunoprecipitated CBX2, NBP1-40342 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for Cbx2 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation Sequencing
1 ug
Immunoprecipitation
2-10 ug/mg lysate
Application Notes
Not recommended for Western Blot.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Cbx2
Long Name
Chromobox Homolog 2
Alternate Names
CDCA6, M33, SRXY5
Gene Symbol
CBX2
Additional Cbx2 Products
Product Documents for Cbx2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cbx2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
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