CBX3 [p Ser83] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-38641

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CBX3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S83 of human CBX3 (NP_009207.2).

Sequence:
RKSLS

Modification

p Ser 83

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CBX3 [p Ser83] Antibody - BSA Free

CBX3 [p Ser83] Antibody

Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] -

Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from NIH/3T3 cells, using CBX3 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM) at 37C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
CBX3 [p Ser83] Antibody

Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] -

Western Blot: CBX3 [p Ser83] Antibody [NBP3-38641] - Western blot analysis of lysates from HeLa cells, using CBX3at 1:1000 dilution or HP1 gamma/CBX3 antibody. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 60s.

Applications for CBX3 [p Ser83] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CBX3

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Alternate Names

chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein

Gene Symbol

CBX3

Additional CBX3 Products

Product Documents for CBX3 [p Ser83] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CBX3 [p Ser83] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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