CCDC90B Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-05879
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human CCDC90B fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CCDC90B Antibody - BSA Free
Immunoprecipitation: CCDC90B Antibody - BSA Free [NBP3-05879] -
Immunoprecipitation: CCDC90B Antibody - BSA Free [NBP3-05879] - CCDC90B was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL NBP3-05879 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-05879, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size :26 kDaWestern Blot: CCDC90B Antibody - BSA Free [NBP3-05879] -
Western Blot: CCDC90B Antibody - BSA Free [NBP3-05879] - NBP3-05879 at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: U-251MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:30 kDa Observed band size:26 kDa (We are unsure as to the identity of these extra bands.)Applications for CCDC90B Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCDC90B
Alternate Names
coiled-coil domain containing 90B, mitochondrial
Gene Symbol
Ccdc90b
Additional CCDC90B Products
Product Documents for CCDC90B Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CCDC90B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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