CCL2/MCP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-07034

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Mouse (95%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, ICC/IF (Negative), Immunohistochemistry (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CCL2/MCP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of rat MCP1 (within residues 30-80). [Swiss-Prot# P14844]

Reactivity Notes

Mouse reactivity reported by a customer review.

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CCL2/MCP1 Antibody - BSA Free

Western Blot: CCL2/MCP1 AntibodyBSA Free [NBP1-07034]

Western Blot: CCL2/MCP1 AntibodyBSA Free [NBP1-07034]

Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of recombinant mouse MCP1. Image provided via product review by verified customer.
Western Blot: CCL2/MCP1 AntibodyBSA Free [NBP1-07034]

Western Blot: CCL2/MCP1 AntibodyBSA Free [NBP1-07034]

Western Blot: CCL2/MCP1 Antibody [NBP1-07034] - Analysis of MCP1 in Hela whole cell lysates.

Applications for CCL2/MCP1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

0.5 ug/ml
Application Notes
In Western blot, a band is seen at ~16 kDa. This antibody did not work in IHC with mouse tissue or in ICC.

Reviewed Applications

Read 1 review rated 5 using NBP1-07034 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.42 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCL2/MCP1

MCP-1 (monocyte chemoattracctant protein-1, also called C-C motif chemokine 2 or CCL2) is a secreted protein belonging to intercrine beta (chemokine CC) family and it specifically act as a chemotactic factor to attracts monocytes, CD4+ T cells, NK cells as well as for CCR2 expressing cells, but not neutrophils. Both hematopoietic and non-hematopoietic cells that include monocytes/macrophages, dendritic cells, astrocytes, endothelial cells and fibroblasts express MCP-1, and monocytes/macrophages are the major source of MCP-1 in peripheral blood. Besides, expression at acute inflammation sites, MCP-1 is upregulated in chronic inflammation, including autoimmune disease, atherosclerosis, cancer, and chronic infection. MCP-1 has been implicated in both pro-inflammatory and anti-inflammatory responses, and is a potential intervention point for the treatment of various inflammatory/autoimmune diseases (multiple sclerosis, atherosclerosis, insulin-resistant diabetes, rheumatoid arthritis and lupus nephritis), cancers (breast and prostate cancer) and HIV-1/AIDS.

Alternate Names

C-C motif chemokine 2, chemokine (C-C motif) ligand 2, GDCF-2, HC11Monocyte secretory protein JE, MCAFMonocyte chemotactic and activating factor, MCP1 Monocyte chemotactic protein 1, MCP-1 Small-inducible cytokine A2, MGC9434, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, SCYA2HSMCR30, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, SMC-CF

Gene Symbol

CCL2

Additional CCL2/MCP1 Products

Product Documents for CCL2/MCP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CCL2/MCP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCL2/MCP1 Antibody - BSA Free

Customer Reviews for CCL2/MCP1 Antibody - BSA Free (1)

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Customer Images

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  • Name: David Jeng
    Application: Western Blot
    Sample Tested: Recombinant Mouse MPC1 protein
    Species: Mouse
    Verified Customer | Posted 06/07/2013
    Western Blot analysis of recombinant mouse MCP1
    CCL2/MCP1 Antibody - BSA Free NBP1-07034

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Protocols

View specific protocols for CCL2/MCP1 Antibody - BSA Free (NBP1-07034):

CCL2/MCP1 Antibody:
Procedure Guide for NBP1-07034 - MCP-1 Antibody
Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer
apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of
proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations
and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% NFDM + 1% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10
minutes each.
7. Dilute the rabbit anti-MCP-1 primary antibody (NBP1-07034) in blocking buffer and incubate 1 hour at room
temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10
minutes each.
9. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers
instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as
required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided
it does not interfere with antibody-antigen binding.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CCL2/MCP1 Antibody - BSA Free

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  • Q: Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?

    A: To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.

  • Q: Would you please let me know what buffer I should use for WB with antibody (MCP1 Antibody)?

    A: In western blot, we recommend diluting this antibody in 1% non-fat dry milk in TBST (0.1% tween-20). This is the same diluent we use for our secondary antibody. For blocking, we use 5% non-fat dry milk in TBST for 1 hour at room temperature.

  • Q: Our customer would like to perform IHC with samples from Rat. She has questions regarding antibodies of choice: 1. Is it possible to use NB400-142 for IHC? 2. I am having difficulty of choosing between NBP1-77679 and NBP1-77680. Would you please help suggest? 3. Although it is described as IHC (-) on the product page of NBP1-07034, This antibody did not work in IHC with mouse tissue is also described. How about Rat samples? Is it possible that the antibody can work in IHC with rat tissues?

    A: To answer your questions about NB400-142, this antibody has not been validated for IHC and we cannot guarantee that it will work. Both NBP1-77679 and NBP1-77680 have been validated for use in rat and IHC. The difference between these 2 antibodies is the immunogen as they were raised against different regions of the protein. Please point out the immunogen to your customer and have them decide what would be best for their research. NBP1-07034 does not work in IHC, it is only guaranteed for Western blot in human, mouse and rat. When an application is followed by a (-) I would not recommend using this application in any species.

  • Q: Would you please let me know what buffer I should use for WB with antibody (MCP1 Antibody)?

    A: In western blot, we recommend diluting this antibody in 1% non-fat dry milk in TBST (0.1% tween-20). This is the same diluent we use for our secondary antibody. For blocking, we use 5% non-fat dry milk in TBST for 1 hour at room temperature.

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