Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 5E8-G9-B4

Format

Azide and BSA Free
View all CCR3 Primary Antibodies »

Product Specifications

Immunogen

CCR3

Specificity

Recognises human CCR3, a chemokine receptor highly expressed of eosinophils, as well as Th2-Tcells, basophils, platelets, mast-cells, mononuclear phagocytes, airway epithelial cells, and hematopoietic progenitor cells (CD34+).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Novus Biologicals Rabbit CCR3 Antibody (5E8-G9-B4) - Chimeric - Azide and BSA Free (NBP2-81046) is a recombinant monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CCR3 Antibody (5E8-G9-B4) - Chimeric - Azide and BSA Free

Flow Cytometry: CCR3 Antibody (5E8-G9-B4) - Chimeric - Azide and BSA Free [NBP2-81046]

Flow Cytometry: CCR3 Antibody (5E8-G9-B4) - Chimeric - Azide and BSA Free [NBP2-81046]

Flow Cytometry: CCR3 Antibody (5E8-G9-B4) - Chimeric [NBP2-81046] - Flow-cytometry using anti-CCR3 antibody (5E8-G9-B4) [NBP2-81046]. Human leukocytes were stained with an isotype control (NBP2-52638, panel A) or the rabbit-chimeric version of 5 E8-G9-B4 (NBP2-81046, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications for CCR3 Antibody (5E8-G9-B4) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2b format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CCR3

CKR3, or C-C chemokine receptor type 3, is a G protein-coupled, seven-transmembrane domain receptor protein. The chemokine eotaxin appears to be a highly specific agonist for CKR3, making CKR3 one of the factors responsible for selective recruitment of eosinophils to sites of inflammation (1). It has been shown that more than one chemokine activates CKR3, including eotaxin, RANTES, and MCP-3 (2). Since CKR3 is prominently expressed in eosinophils, it has been named the human eosinophil eotaxin receptor (2,3).

Alternate Names

CCR3, CD193

Gene Symbol

CCR3

Additional CCR3 Products

Product Documents for CCR3 Antibody (5E8-G9-B4) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CCR3 Antibody (5E8-G9-B4) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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