CD11b/c Antibody [FITC]

Novus Biologicals | Catalog # NB110-40766F

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

A synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein. [Swiss-Prot: P05555]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25150592)

Localization

Monocytes and granulocytes.

Marker

Microglia Marker, Macrophage Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for CD11b/c Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB110-40766F in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD11b/c

CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.

Alternate Names

antigen CD11b (p170), CD11 antigen-like family member B, CD11b, CD11b antigen, CD11Bintegrin, alpha M (complement component receptor 3, alpha; also known as CD11b(p170), macrophage antigen alpha polypeptide), Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3AMGC117044, integrin alpha-M, integrin, alpha M (complement component 3 receptor 3 subunit), Leukocyte adhesion receptor MO1, MAC-1, MAC1A, macrophage antigen alpha polypeptide, MO1A, Neutrophil adherence receptor, neutrophil adherence receptor alpha-M subunit, SLEB6

Gene Symbol

ITGAM

Additional CD11b/c Products

Product Documents for CD11b/c Antibody [FITC]

Certificate of Analysis

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Product Specific Notices for CD11b/c Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD11b/c Antibody [FITC] (1)

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  • CD11b/c Antibody [FITC]
    Name: Jiapeng Wang
    Application: Immunocytochemistry
    Sample Tested: N9 mouse microglial cell line
    Species: Mouse
    Verified Customer | Posted 08/23/2017
    CD11b/c Antibody [FITC] NB110-40766F

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