Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B-E31
View all CD151 Primary Antibodies »

Product Specifications

Immunogen

Eahy 926 cell line. Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cell

Specificity

Recognizes the antigen PETA-3, a 32 kDa protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for CD151 Antibody (B-E31) [CoraFluor™ 1]

CD151 Antibody (B-E31) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for CD151 Antibody (B-E31) [CoraFluor™ 1]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD151

CD151 is essential for the proper assembly of the glomerular and tubular basement membranes in kidney. It interacts with integrins alpha3beta1, alpha5beta1, alpha3beta1 and alpha6beta4, and with CD9 and CD181. It is expressed in a variety of tissues including vascular endothelium and epidermis, platelets, monocytes and also erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. CD151 is however not expressed by lymphocytes or granulocytes. Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness.

Alternate Names

CD151, PETA-3, SFA-1, TSPAN24

Gene Symbol

CD151

Additional CD151 Products

Product Documents for CD151 Antibody (B-E31) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD151 Antibody (B-E31) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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