CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20165

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Flow Cytometry, Cell depletion

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # PK-136

Format

Azide and BSA Free
View all CD161/NK1.1 Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing (C3H X BALB)F1 mice with spleen cells enriched for NK-1 cells and bone marrow cells from CE mice.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] -

Immunocytochemistry /Immunofluorescence: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with NBP3-20165 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20165, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Flow Cytometry: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] -

Flow Cytometry: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-20165 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Western Blot: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] -

Western Blot: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - RAW264.7(A) (1ug/ml) cell lysate, mouse spleen(B) (1ug/ml) and mouse lymph node(C) (0.3ug/ml) tissue lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP3-20165 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Applications for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Cell depletion

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD161/NK1.1

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Alternate Names

Klrb1c, Ly-59, Ly55c

Gene Symbol

Klrb1c

Additional CD161/NK1.1 Products

Product Documents for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

There are currently no reviews for this product. Be the first to review CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free and earn rewards!

Have you used CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies