CD1d Antibody (1B1) - BSA Free
Novus Biologicals | Catalog # NBP1-43461
![Flow Cytometry: CD1d Antibody (1B1) - BSA Free [NBP1-43461]](https://resources.rndsystems.com/images/products/CD1d-Antibody-1B1-Flow-Cytometry-NBP1-43461-img0003.jpg "Flow Cytometry: CD1d Antibody (1B1) - BSA Free [NBP1-43461]")
Key Product Details
Species Reactivity
Mouse, Hamster
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # 1B1
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this antibody was CD1d.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Scientific Data Images for CD1d Antibody (1B1) - BSA Free
Flow Cytometry: CD1d Antibody (1B1) - BSA Free [NBP1-43461]
Flow Cytometry: CD1d Antibody (1B1) [NBP1-43461] - Analysis using the Biotin conjugate of NBP1-43461. Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.5 ug of Rat IgG2b kappa Isotype Control Biotin (left) or 0.5 ug of Anti-Mouse CD1d Biotin (right) followed by Streptavidin PE.![Flow Cytometry: CD1d Antibody (1B1) - BSA Free [NBP1-43461]](https://resources.rndsystems.com/images/products/CD1d-Antibody-1B1-Flow-Cytometry-NBP1-43461-img0001.jpg "Flow Cytometry: CD1d Antibody (1B1) - BSA Free [NBP1-43461]")
Flow Cytometry: CD1d Antibody (1B1) - BSA Free [NBP1-43461]
Flow Cytometry: CD1d Antibody (1B1) [NBP1-43461] - Staining of BALB/c splenocytes with 1.0 ug of Rat IgG2b Isotype Control Purified (open histogram) or 1.0 ug of Anti-Mouse CD1d Purified (filled histogram) followed by Anti-Rat IgG FITC. Total viable cells were used for analysis.Applications for CD1d Antibody (1B1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
This product has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD1d
Alternate Names
CD1d, R3, R3G1
Gene Symbol
CD1D
Additional CD1d Products
Product Documents for CD1d Antibody (1B1) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD1d Antibody (1B1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CD1d Antibody (1B1) - BSA Free
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Q: Do you have an antibody for CD1d that works well on formalin fixed paraffin embedded tissue (specifically for liver tissue)?
A:
We do have a CD1d antibody that has been verified in paraffin-embedded tissue sections. The catalog number is NBP1-43461.
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