CD1d Antibody (51.1) - BSA Free

Novus Biologicals | Catalog # NBP1-43460

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 51.1

Format

BSA Free
View all CD1d Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD1d.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CD1d Antibody (51.1) - BSA Free

Flow Cytometry: CD1d Antibody (51.1) - BSA Free [NBP1-43460]

Flow Cytometry: CD1d Antibody (51.1) - BSA Free [NBP1-43460]

Flow Cytometry: CD1d Antibody (51.1) [NBP1-43460] - Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG2b K Isotype Control Purified (open histogram) or Anti-Human CD1d Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Cells in the lymphocyte gate were

Applications for CD1d Antibody (51.1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This 51.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD1d

The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family with similarity to the non-polymorphic MHC Class I-like molecules. CD1d is a highly conserved single transmembrane receptor of the Immunoglobulin Superfamily. CD1d can associate with beta-microglobulin another feature showing similarity to MHC class I molecules, but can also exist as a nonglycosylated protein not in association with beta microglobulin. This suggests different control mechanisms for presenting glycolipid containing molecules to CD1d reactive NKT cells. Expression of CD1d is found on B cells of the periphery, in resting monocytes and cortical thymocytes. On intestinal epithelial cells (IEC) expression is polarized. Expression can also be found at low levels intracellularly in hepatocytes. In HCV (hepatitis C virus) livers, CD1d is highly expressed compared to normal controls.

Alternate Names

CD1d, R3, R3G1

Gene Symbol

CD1D

Additional CD1d Products

Product Documents for CD1d Antibody (51.1) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD1d Antibody (51.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for CD1d Antibody (51.1) - BSA Free

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  • Q: Do you have an antibody for CD1d that works well on formalin fixed paraffin embedded tissue (specifically for liver tissue)?

    A:

    We do have a CD1d antibody that has been verified in paraffin-embedded tissue sections. The catalog number is NBP1-43461.

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