CD23/Fc epsilon RII Antibody (SP23)

Novus Biologicals | Catalog # NB120-16702

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # SP23
View all CD23/Fc epsilon RII Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment, corresponding to amino acids 48-248 of Human CD23.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Localization

Cell Membrane

Specificity

This antibody recognizes CD23, a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells. It is strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukemia and some cases of centroblastic/centrocytic lymphoma.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD23/Fc epsilon RII Antibody (SP23)

Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (SP23) [NB120-16702]

Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (SP23) [NB120-16702]

Immunohistochemistry-Paraffin: CD23/Fc epsilon RII Antibody (SP23) [NB120-16702] - Formalin fixed paraffin embedded human tonsil stained with CD23 antibody (NB120-16702).

Applications for CD23/Fc epsilon RII Antibody (SP23)

Application
Recommended Usage

Immunohistochemistry

1:25-1:50

Immunohistochemistry-Paraffin

1:25-1:50
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30-60 min at RT.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD23/Fc epsilon RII

The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets and EBV transformed B lymphoblasts. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia. CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils. CD23 is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a

Long Name

Fc epsilon Receptor II

Alternate Names

CD23, CLEC4J, Fc epsilon RII, FCER2, Fcer2a, FceRII, IGEBF, Ly-42

Entrez Gene IDs

2208 (Human)

Gene Symbol

FCER2

Additional CD23/Fc epsilon RII Products

Product Documents for CD23/Fc epsilon RII Antibody (SP23)

Certificate of Analysis

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Product Specific Notices for CD23/Fc epsilon RII Antibody (SP23)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD23/Fc epsilon RII Antibody (SP23)

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Protocols

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