CD3 gamma Antibody (5B7B2) - BSA Free

Novus Biologicals | Catalog # NBP2-61729

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B7B2

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human CD3 gamma (AA: extra 23-116) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD3 gamma Antibody (5B7B2) - BSA Free

Western Blot: CD3 gamma Antibody (5B7B2)BSA Free [NBP2-61729]

Western Blot: CD3 gamma Antibody (5B7B2)BSA Free [NBP2-61729]

Western Blot: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Analysis using CD3G mAb against HEK293 (1) and CD3G (AA: extra 23-116)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: CD3 gamma Antibody (5B7B2) - BSA Free [NBP2-61729]

Flow Cytometry: CD3 gamma Antibody (5B7B2) - BSA Free [NBP2-61729]

Flow Cytometry: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Analysis of Raji cells using CD3G mouse mAb (green) and negative control (red).
Western Blot: CD3 gamma Antibody (5B7B2)BSA Free [NBP2-61729]

Western Blot: CD3 gamma Antibody (5B7B2)BSA Free [NBP2-61729]

Western Blot: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Analysis using CD3G mAb against human CD3G (AA: extra 23-116) recombinant protein. (Expected MW is 36.6 kDa)
Western Blot: CD3 gamma Antibody (5B7B2)BSA Free [NBP2-61729]

Western Blot: CD3 gamma Antibody (5B7B2)BSA Free [NBP2-61729]

Western Blot: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Analysis using CD3G mouse mAb against Jurkat (1) cell lysate.
ELISA: CD3 gamma Antibody (5B7B2) - BSA Free [NBP2-61729]

ELISA: CD3 gamma Antibody (5B7B2) - BSA Free [NBP2-61729]

ELISA: CD3 gamma Antibody (5B7B2) [NBP2-61729] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for CD3 gamma Antibody (5B7B2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Western Blot

1:100-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 gamma

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.

Alternate Names

CD3g, IMD17, T3G

Gene Symbol

CD3G

Additional CD3 gamma Products

Product Documents for CD3 gamma Antibody (5B7B2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD3 gamma Antibody (5B7B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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