CD30/TNFRSF8 Antibody [ADC] - (Brentuximab Vedotin, Research Grade)

Novus Biologicals | Catalog # NBP3-44512

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

ADC

Antibody Source

Monoclonal Human IgG1
View all CD30/TNFRSF8 Primary Antibodies »

Product Specifications

Immunogen

CD30/TNFRSF8 (Uniprot: P28908)

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Theoretical MW

145.48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This product is an Antibody-Drug Conjugate (ADC) for Research Use Only (RUO).

Brentuximab vedotin is an ADC consisting of a CD30-directed antibody and Monomethyl auristatin E (MMAE) that inhibits CD30-positive cells and is used for the study of relapsed and refractory Hogkin's lymphoma.

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD30/TNFRSF8 Antibody [ADC] - (Brentuximab Vedotin, Research Grade)

Hu CD30/TNFRSF8 mAb [ADC] - (Brentuximab Vedotin)

ELISA: Hu CD30/TNFRSF8 mAb [ADC] - (Brentuximab Vedotin) [NBP3-44512] -

ELISA: CD30/TNFRSF8 (Brentuximab Vedotin) Antibody [NBP3-44512] - Immobilized human CD30 His at 2 ug/mL can bind CD30/TNFRSF8 (Brentuximab Vedotin) Antibody, EC50=0.006007 ug/mL.

Applications for CD30/TNFRSF8 Antibody [ADC] - (Brentuximab Vedotin, Research Grade)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

> 90% by SDS-PAGE and HPLC

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD30/TNFRSF8

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Alternate Names

CD30, TNFRSF8

Additional CD30/TNFRSF8 Products

Product Documents for CD30/TNFRSF8 Antibody [ADC] - (Brentuximab Vedotin, Research Grade)

Certificate of Analysis

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Product Specific Notices for CD30/TNFRSF8 Antibody [ADC] - (Brentuximab Vedotin, Research Grade)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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