CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647]

Novus Biologicals | Catalog # NB100-1642AF647

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vivo assay

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # MEC 7.46
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

This CD31/PECAM-1 Antibody (MEC 7.46) was developed against mouse endothelial cell line T-end.

Localization

Membrane; Single-pass type I membrane protein. Cell junction.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647] [NB100-1642AF647] - CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor(R) 647] [NB100-1642AF647] - Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature.
Flow Cytometry: CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647] [NB100-1642AF647] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC 7.46] Antibody NB100-1642AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647]

CD31/PECAM-1 (MEC 7.46) in MS1 Mouse Cell Line.

CD31/PECAM-1 (MEC 7.46) was detected in immersion fixed MS1 mouse pancreas/Islet of Langerhans endothelial cell line using Rat anti-CD31/PECAM-1 (MEC 7.46) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-1642AF647) (light blue) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647]

Application
Recommended Usage

Flow (Cell Surface)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

In vivo assay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-1642AF647 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647] (2)

4.5 out of 5
2 Customer Ratings
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  • CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647]
    Name: James Xiao
    Application: Flow Cytometry
    Sample Tested: IL1R-/- KO Mouse lung
    Species: Mouse
    Verified Customer | Posted 03/14/2018
    Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets.
    The single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647] at the dilution of 1:100 for 30 minutes. Cells were analyzed by flow cytometer.
    CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647] NB100-1642AF647
  • CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647]
    Name: James Xiao
    Application: Immunohistochemistry-Frozen
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 03/02/2018
    B6 mouse, both WT and IL1R KO were used. Green: CD31/PECAM-1. Red: F4/80. Blue: DAPI+ nuclei
    Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with Alex647-conjugated CD31/PECAM-1 antiboady (MEC 7.46) and PE-conjugated F4/80 for 2 hours at the room temperature.
    CD31/PECAM-1 Antibody (MEC 7.46) [Alexa Fluor® 647] NB100-1642AF647

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