CD35 Antibody (CR1/802) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48365

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Baboon, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CR1/802

Format

IHC-Prediluted
View all CD35 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CD35 protein (Uniprot: P17927)

Localization

Cell surface

Marker

Follicular Dendritic Cell Marker

Specificity

Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for CD35 Antibody (CR1/802) - IHC-Prediluted

Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - IHC-Prediluted [NBP2-48365]

Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - IHC-Prediluted [NBP2-48365]

Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - IHC-Prediluted [NBP2-48365] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Mouse Monoclonal Antibody (CR1/802).
Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - IHC-Prediluted [NBP2-48365]

Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - IHC-Prediluted [NBP2-48365]

Immunohistochemistry-Paraffin: CD35 Antibody (CR1/802) - IHC-Prediluted [NBP2-48365] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Mouse Monoclonal Antibody (CR1/802).

Applications for CD35 Antibody (CR1/802) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD35

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Alternate Names

C3BR, CD35, CR1, KN

Gene Symbol

CR1

Additional CD35 Products

Product Documents for CD35 Antibody (CR1/802) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for CD35 Antibody (CR1/802) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

B Cell Activation
Complement Pathway

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Protocols

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