CD35 Antibody (MSVA-035M) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57344

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSVA-035M

Format

Azide and BSA Free
View all CD35 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human CD35 fragment Swissprot P17927

Localization

Membranous

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Tonsil: A strong CD35 must be seen in follicular dendritic cells while there is a less intense CD35 staining of a subset of lymphocytes.

Negative Control
Negitve control:Tonsil: Squamous epithelium and most lymphocytic cells must be CD35 negative.

Scientific Data Images for CD35 Antibody (MSVA-035M) - Azide and BSA Free

CD35 Antibody (MSVA-035M) - Azide and BSA Free

Immunohistochemistry-Paraffin:CD35 Antibody (MSVA-035M) - Azide and BSA FreeNBP3-57344

Appendix mucosa with strong membranous CD35 staining of follicular dendritic cells and a moderate to strong CD35 positivity of a large subset of lymphocytes. The epithelium is CD35 negative
CD35 Antibody (MSVA-035M) - Azide and BSA Free

Immunohistochemistry-Paraffin:CD35 Antibody (MSVA-035M) - Azide and BSA FreeNBP3-57344

Kidney with strong membranous CD35 positivity of visceral podocytes and peripheral cells of the Bowman capsule.
CD35 Antibody (MSVA-035M) - Azide and BSA Free

Immunohistochemistry-Paraffin:CD35 Antibody (MSVA-035M) - Azide and BSA FreeNBP3-57344

Marginal zone lymphoma with distinct CD35 staining of tumor cells.

Applications for CD35 Antibody (MSVA-035M) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD35

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Alternate Names

C3BR, CD35, CR1, KN

Gene Symbol

CR1

Additional CD35 Products

Product Documents for CD35 Antibody (MSVA-035M) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD35 Antibody (MSVA-035M) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

B Cell Activation
Complement Pathway

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Protocols

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