Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # A1
View all CD39/ENTPD1 Primary Antibodies »

Product Specifications

Immunogen

PHA activated human lymphocytes

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD39/ENTPD1 Antibody (A1)

CD39/ENTPD1 Antibody (A1) Detection of CD39/ENTPD1 (A1) in Human PBMC lymphocytes by Flow Cytometry.

Detection of CD39/ENTPD1 (A1) in Human PBMC lymphocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with CD19 Monoclonal Antibody and either (top) Mouse Anti-Human CD39 (catalog #NBP3-48409) or (bottom) matched isotype control antibody.
CD39/ENTPD1 Antibody (A1) CD39/ENTPD1 Antibody (A1) Detection of CD39 in Human Spleen.

Detection of CD39 in Human Spleen.

CD39 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD39 Monoclonal Antibody (Catalog #NBP3-48409) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Applications for CD39/ENTPD1 Antibody (A1)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD39/ENTPD1

CD39, also known as ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1), is the prototypical member of the NTPDase family. CD39 is a metal-dependent, homodimeric enzyme bound to the cell membrane through two transmembrane domains present at each termini (1-3). The extracellular loop contains two major domains with an active site and apyrase conserved regions (ACRs) and changes conformation dynamically during catalysis (1-4). Although first described as a B lymphocyte cell surface marker (5), CD39 is also present on the surface of natural killer cells, T cells, and some endothelial cells (6). CD39 hydrolyzes the beta - and gamma  phosphate residues of nucleotides to terminate purinergic signaling and is uniquely capable of processive hydrolysis of extracellular ATP to AMP without release of the ADP intermediate (3, 7, 8) as the initial rate-limiting steps in the production of extracellular adenosine (9). CD39 functional activity leads to interest in CD39 as a pharmaceutical therapeutic target for several disease models including inflammation, cancer, and immunosuppression (3,4,9,10). Recombinant cynomolgus NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble recombinant NTPDase-1 ectodomain.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 1

Alternate Names

ADPase, ATP-diphosphatase, CD39, ENTPD1, NTPD1

Gene Symbol

ENTPD1

Additional CD39/ENTPD1 Products

Product Documents for CD39/ENTPD1 Antibody (A1)

Certificate of Analysis

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Product Specific Notices for CD39/ENTPD1 Antibody (A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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