CD42b/GPIb alpha Antibody (AK2) - BSA Free

Novus Biologicals | Catalog # NB100-63790

Clone AK2 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AK2

Format

BSA Free
View all CD42b/GPIb alpha Primary Antibodies »

Product Specifications

Immunogen

Made to Human CD42b

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:29437579).

Specificity

Recognises the human CD42b cell surface antigen, also known as platelet glycoprotein GP1B. CD42b is expressed by platelets and megakaryocytes. Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets (1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD42b/GPIb alpha Antibody (AK2) - BSA Free

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the FITC conjugate of NB100-63790. Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b:FITC.
Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with Mouse anti Human CD42b.
Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.
Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.
Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b: RPE.
Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the Biotin conjugate of NB100-63790. Staining of human peripheral blood platelets.
Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - After labeling the surface proteins of the CHO a beta IX cells with biotin, the GPIbIX complex was immunoprecipitated using the MoAb panel. The presence of the GPIba and GPIX native proteins was shown on the surface of the CHO a beta IX cells. Lane 1 shows the components immunoprecipitated using AK3, lane 2 with AK2, and lane 3 with SZ2. After cleavage with mocarhagin, AK3 precipitated a 70-kD GPIba component and the intact GPIX protein from the surface of the cells (lane 4). AK2 (lane 5) and SZ2 (lane 6) were able to precipitate a 40-kD GPIba component from the supernatant collected after the cleavage step.

Applications for CD42b/GPIb alpha Antibody (AK2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:50-1:100

Immunoprecipitation

1:10-1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD42b/GPIb alpha

CD42a is a small membrane glycoprotein found on the surface of human platelets. It forms a one to one noncovalent complex with glycoprotein Ib, a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain and a beta chain, that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein V. CD42a is present on thrombocytes and also on a so far unidentified subpopulation of T lymphocytes.

Long Name

Glycoprotein lb [Platelet] alpha

Alternate Names

BP1BA, BSS, CD42b, GP1BA, GPIb alpha

Gene Symbol

GP1BA

UniProt

P07359

Additional CD42b/GPIb alpha Products

Product Documents for CD42b/GPIb alpha Antibody (AK2) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD42b/GPIb alpha Antibody (AK2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD42b/GPIb alpha Antibody (AK2) - BSA Free

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Protocols

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FAQs

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