CD58/LFA-3 Antibody (085) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-90096

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 085 expressed in HEK293

Format

Azide and BSA Free
View all CD58/LFA-3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD58/LFA-3 (Accession#: AAH05930.1; Met1-Arg215).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD58/LFA-3 Antibody (085) - Azide and BSA Free

Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096]

Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096]

Western Blot: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A: Jurkat Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: CD58/LFA-3 Antibody (085) [NBP2-90096]

Immunoprecipitation: CD58/LFA-3 Antibody (085) [NBP2-90096]

Immunoprecipitation: CD58/LFA-3 Antibody (085) [NBP2-90096] - Lane A:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-CD58 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD58 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 28 kDaObserved band size: 61 kDa

Applications for CD58/LFA-3 Antibody (085) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD58/LFA-3

LFA3 is a type 1 transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed on most hematopoietic cells and on nonhematopoietic cells such as fibroblasts, endothelial and epithelial cells. Its expression is particularly high on memory T-cells and dendritic cells. LFA3 expression on antigen-presenting cells and target cells enhances T-cells antigen recognition through binding to its ligand, LFA2/CD2, on T-cells.

Alternate Names

CD58, LFA-3, LFA3

Gene Symbol

CD58

Additional CD58/LFA-3 Products

Product Documents for CD58/LFA-3 Antibody (085) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD58/LFA-3 Antibody (085) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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