CD58/LFA-3 Antibody (PSH02-74)

Western Blot: CD58/LFA-3 Antibody (PSH02-74) [NBP3-32161]

Western Blot: CD58/LFA-3 Antibody (PSH02-74) [NBP3-32161] - Western blot analysis of CD58/LFA-3 on different lysates with Rabbit anti-CD58/LFA-3 antibody (NBP3-32161) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Raji cell lysate
Lane 3: Jurkat cell lysate
Lane 4: K-562 cell lysate
Lane 5: HUVEC cell lysate
Lane 6: HT-29 cell lysate
Lane 7: TF-1 cell lysate
Lane 8: MCF7 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 28 kDa
Observed band size: 55 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32161) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: CD58/LFA-3 Antibody (PSH02-74) [NBP3-32161]

Flow Cytometry: CD58/LFA-3 Antibody (PSH02-74) [NBP3-32161] - Flow cytometric analysis of human peripheral blood lymphocytes labeling CD58/LFA-3.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32161, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (black). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.