CD6 Antibody (SPM547) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-44555

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM547

Format

IHC-Prediluted
View all CD6 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant CD6 protein (Uniprot: P30203)

Localization

Cell surface and Cytoplasmic

Marker

Negative Marker of T-regulatory Cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for CD6 Antibody (SPM547) - IHC-Prediluted

Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) - IHC-Prediluted [NBP2-44555]

Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) - IHC-Prediluted [NBP2-44555]

Immunohistochemistry-Paraffin: CD6 Antibody (SPM547) - IHC-Prediluted [NBP2-44555] - Formalin-fixed paraffin-embedded human tonsil stained with CD6 Monoclonal antibody (SPM547).
CD6 Antibody (SPM547) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPM547) - IHC-Prediluted [NBP2-44555] -

Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPM547) - IHC-Prediluted [NBP2-44555] -

Immunofluorescence staining of MOLT-4 cells using CD6 Antibody (SPM547) - IHC-Prediluted followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Applications for CD6 Antibody (SPM547) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD6

CD6 is a 100-130 kD single chain transmembrane glycoprotein also known as T12. It is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6, interacting with its ligand CD166 (also known as ALCAM), is involved in T cell development and activation, as well as thymocyte adhesion.

Alternate Names

CD6

Gene Symbol

CD6

Additional CD6 Products

Product Documents for CD6 Antibody (SPM547) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for CD6 Antibody (SPM547) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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