CD6 Antibody (SPV-L14)
Novus Biologicals | Catalog # NBP2-44556
Clone SPV-L14 was used by HLDA to establish CD designation.
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPV-L14
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Product Specifications
Immunogen
CD8+ cytotoxic T-cell clone
Localization
Cell surface and cytoplasmic
Marker
Negative Marker of T-regulatory Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11571)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD6 Antibody (SPV-L14)
![Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPV-L14) [NBP2-44556]](https://beta-resources.rndsystems.com/images/products/CD6-Antibody-SPV-L14-Immunocytochemistry-Immunofluorescence-NBP2-44556-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPV-L14) [NBP2-44556]")
Immunocytochemistry/ Immunofluorescence: CD6 Antibody (SPV-L14) [NBP2-44556]
Immunocytochemistry/Immunofluorescence: CD6 Antibody (SPV-L14) [NBP2-44556] - Immunofluorescence staining of MOLT4 cells using CD6 Antibody (SPV-L14) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.![CD6 Antibody (SPV-L14) Flow Cytometry: CD6 Antibody (SPV-L14) [NBP2-44556] -](https://beta-resources.rndsystems.com/images/products/nbp2-44556_mouse-monoclonal-cd6-antibody-spv-l14-295202516395335.jpg "Flow Cytometry: CD6 Antibody (SPV-L14) [NBP2-44556] -")
Flow Cytometry: CD6 Antibody (SPV-L14) [NBP2-44556] -
Flow Cytometric Analysis of MOLT-4 cells. CD6 Antibody (SPV-L14) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).Applications for CD6 Antibody (SPV-L14)
Application
Recommended Usage
Flow Cytometry
1-2 ug/ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD6
Additional CD6 Products
Product Documents for CD6 Antibody (SPV-L14)
Product Specific Notices for CD6 Antibody (SPV-L14)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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