CD74 Antibody (LN-2) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-44988

Clone LN-2 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human, Mouse, Baboon, Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LN-2

Format

IHC-Prediluted
View all CD74 Primary Antibodies »

Product Specifications

Immunogen

SU-DHL-4 lymphoma cells

Reactivity Notes

Does not react with Rat.

Localization

Cell surface and paranuclear globular

Marker

B-Cell Marker

Specificity

It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for CD74 Antibody (LN-2) - IHC-Prediluted

Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988]

Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988]

Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Antibody (LN-2).
Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988]

Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988]

Immunohistochemistry-Paraffin: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988] - Formalin-fixed, paraffin-embedded human Spleen stained with CD74 Antibody (LN-2).
CD74 Antibody (LN-2) - IHC-Prediluted Flow Cytometry: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988] -

Flow Cytometry: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988] -

Flow Cytometric Analysis of Human Raji cells using CD74 Antibody (LN-2) - IHC-Prediluted followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).
CD74 Antibody (LN-2) - IHC-Prediluted Western Blot: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988] -

Western Blot: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988] -

Western blot analysis of Raji cell lysate using CD74 Antibody (LN-2) - IHC-Prediluted.
CD74 Antibody (LN-2) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988] -

Immunocytochemistry/ Immunofluorescence: CD74 Antibody (LN-2) - IHC-Prediluted [NBP2-44988] -

Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (LN-2) - IHC-Prediluted followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)

Applications for CD74 Antibody (LN-2) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD74

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41 kDa, depending on genetic splicing.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Gene Symbol

CD74

Additional CD74 Products

Product Documents for CD74 Antibody (LN-2) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for CD74 Antibody (LN-2) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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