CD8 beta Antibody (H35-17.2) - BSA Free

Novus Biologicals | Catalog # NBP1-43449

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2b Kappa Clone # H35-17.2

Format

BSA Free
View all CD8 beta Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD8.

Reactivity Notes

This antibody is reactive to Mouse.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Scientific Data Images for CD8 beta Antibody (H35-17.2) - BSA Free

Flow Cytometry: CD8 beta Antibody (H35-17.2) - BSA Free [NBP1-43449]

Flow Cytometry: CD8 beta Antibody (H35-17.2) - BSA Free [NBP1-43449]

Flow Cytometry: CD8 beta Antibody (H35-17.2) [NBP1-43449] - Analysis using the FITC conjugate of NBP1-43449. Staining of BALB/c splenocytes with Anti-Mouse CD8a APC and 0.125 ug of Rat IgG2b k Isotype Control FITC (left) or 0.125 ug of Anti-Mouse CD8b FITC (right). Cells in the lymphocyte gate were used for analysis,
Flow Cytometry: CD8 beta Antibody (H35-17.2) - BSA Free [NBP1-43449]

Flow Cytometry: CD8 beta Antibody (H35-17.2) - BSA Free [NBP1-43449]

Flow Cytometry: CD8 beta Antibody (H35-17.2) [NBP1-43449] - Staining of BALB/c splenocytes with 0.25 ug of Rat IgG2b Isotype Control Purified (open histogram) or 0.25 ug of Purified Anti-Mouse CD8b Purified (filled histogram) followed by Anti-Rat IgG FITC. Cells in the lymphocyte gate were used for analysis.

Applications for CD8 beta Antibody (H35-17.2) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 ug/10^6 cells in 100 ul volume

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This H35-17.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD8 beta

The H35-17.2 monoclonal antibody reacts with the mouse CD8beta molecule. The CD8beta chain associates with the CD8alpha chain to form the CD8alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alphabeta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Alternate Names

CD8 beta, CD8b, CD8B1, Leu2, LY3, LYT3, P37

Entrez Gene IDs

12526 (Mouse)

Gene Symbol

CD8B1

UniProt

P10300 (Mouse)

Additional CD8 beta Products

Product Documents for CD8 beta Antibody (H35-17.2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD8 beta Antibody (H35-17.2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for CD8 beta Antibody (H35-17.2) - BSA Free

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  • Q: May we ask the suggested dilution titers while using NB200-578 and NBP1-43449 for IHC?

    A: A good starting point for optimization in IHC for both products would be 1:100.

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