CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-62563

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # YTS 156.7.7

Format

Azide and BSA Free
View all CD8 beta Primary Antibodies »

Product Specifications

Immunogen

Mouse CD8 (lyt-3)

Specificity

This CD8 Antibody (YTS 156.7.7) - Chimeric recognises the B epitope of the mouse Lyt-3 (CD8 beta) antigen. Has been used in CD8 subset depletion in vivo and flow cytometry studies.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free

Flow Cytometry: CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free [NBP2-62563]

Flow Cytometry: CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free [NBP2-62563]

Flow Cytometry: CD8 beta Antibody (YTS 156.7.7) [NBP2-62563] - Mouse thymocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of YTS 156.7.7 (NBP2-62563, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
Flow Cytometry: CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free [NBP2-62563]

Flow Cytometry: CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free [NBP2-62563]

Flow Cytometry: CD8 beta Antibody (YTS 156.7.7) [NBP2-62563] - Mouse lymphocytes were stained with an anti-CD44 control (panel A) or the rabbit-chimeric version of YTS 156.7.7 (NBP2-62563, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications for CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD8 beta

CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8 beta. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8 alpha chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Alternate Names

CD8 beta, CD8b, CD8B1, Leu2, LY3, LYT3, P37

Gene Symbol

CD8B

Additional CD8 beta Products

Product Documents for CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD8 beta Antibody (YTS 156.7.7) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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