![Western Blot: CD9 Antibody (5G6)BSA Free [NBP2-22187]](https://beta-resources.rndsystems.com/images/products/CD9-Antibody-5G6-Western-Blot-NBP2-22187-img0005.jpg "Western Blot: CD9 Antibody (5G6)BSA Free [NBP2-22187]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunoassay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5G6
Loading...
Product Specifications
Immunogen
Synthesized peptide of human CD9.
Localization
Cytoplasm, Membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse CD9 Antibody (5G6) (NBP2-22187) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-CD9 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD9 Antibody (5G6)
Western Blot: CD9 Antibody (5G6)BSA Free [NBP2-22187]
Western Blot: CD9 Antibody (5G6) [NBP2-22187] - Analysis using CD9 mAb against HEK293 (1) and CD9(AA: 37-228)-hIgGFc transfected HEK293 (2) cell lysate.![Immunocytochemistry/ Immunofluorescence: CD9 Antibody (5G6) - BSA Free [NBP2-22187]](https://beta-resources.rndsystems.com/images/products/CD9-Antibody-5G6-Immunofluorescence-NBP2-22187-img0006.jpg "Immunocytochemistry/ Immunofluorescence: CD9 Antibody (5G6) - BSA Free [NBP2-22187]")
Immunocytochemistry/ Immunofluorescence: CD9 Antibody (5G6) - BSA Free [NBP2-22187]
Immunocytochemistry/Immunofluorescence: CD9 Antibody (5G6) [NBP2-22187] - Human endometrial stromal cells stained with anti-CD9 antibody and detected with anti-mouse IgG AlexaFluor 488 secondary antibody (Green). Image submitted by a verified customer review.![Immunohistochemistry-Paraffin: CD9 Antibody (5G6) - BSA Free [NBP2-22187]](https://beta-resources.rndsystems.com/images/products/CD9-Antibody-5G6-Immunohistochemistry-Paraffin-NBP2-22187-img0003.jpg "Immunohistochemistry-Paraffin: CD9 Antibody (5G6) - BSA Free [NBP2-22187]")
Immunohistochemistry-Paraffin: CD9 Antibody (5G6) - BSA Free [NBP2-22187]
Immunohistochemistry-Paraffin: CD9 Antibody (5G6) [NBP2-22187] - Analysis of kidney cancer tissues using CD9 mouse mAb with DAB staining.![Flow Cytometry: CD9 Antibody (5G6) - BSA Free [NBP2-22187]](https://beta-resources.rndsystems.com/images/products/CD9-Antibody-5G6-Flow-Cytometry-NBP2-22187-img0001.jpg "Flow Cytometry: CD9 Antibody (5G6) - BSA Free [NBP2-22187]")
Flow Cytometry: CD9 Antibody (5G6) - BSA Free [NBP2-22187]
Flow Cytometry: CD9 Antibody (5G6) [NBP2-22187] - Analysis of Jurkat cells using CD9 mouse mAb (green) and negative control (red).![Immunohistochemistry-Paraffin: CD9 Antibody (5G6) - BSA Free [NBP2-22187]](https://beta-resources.rndsystems.com/images/products/CD9-Antibody-5G6-Immunohistochemistry-Paraffin-NBP2-22187-img0002.jpg "Immunohistochemistry-Paraffin: CD9 Antibody (5G6) - BSA Free [NBP2-22187]")
Immunohistochemistry-Paraffin: CD9 Antibody (5G6) - BSA Free [NBP2-22187]
Immunohistochemistry-Paraffin: CD9 Antibody (5G6) [NBP2-22187] - Analysis of cervical cancer tissues using CD9 mouse mAb with DAB staining.![ELISA: CD9 Antibody (5G6) - BSA Free [NBP2-22187]](https://beta-resources.rndsystems.com/images/products/CD9-Antibody-5G6-ELISA-NBP2-22187-img0004.jpg "ELISA: CD9 Antibody (5G6) - BSA Free [NBP2-22187]")
ELISA: CD9 Antibody (5G6) - BSA Free [NBP2-22187]
ELISA: CD9 Antibody (5G6) [NBP2-22187] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)Applications for CD9 Antibody (5G6)
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
Validated from a verified customer review
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Reviewed Applications
Read 1 review rated 5 using NBP2-22187 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD9
Alternate Names
CD9, DRAP-27, MIC3, p24, TSPAN29
Gene Symbol
CD9
Additional CD9 Products
Product Documents for CD9 Antibody (5G6)
Product Specific Notices for CD9 Antibody (5G6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CD9 Antibody (5G6)
Powered by Bioz
Customer Reviews for CD9 Antibody (5G6) (1)
5 out of 5
1 Customer Rating
Have you used CD9 Antibody (5G6)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: ImmunofluorescenceSample Tested: Human endometrial stromal cellsSpecies: HumanVerified Customer | Posted 01/18/2016Human endometrial stromal cells stained with anti-CD9 antibody and anti-mouse IgG AlexaFluor 488 secondary
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...