Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all CD97 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CD97

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD97 Antibody - Azide and BSA Free

Western Blot: CD97 Antibody [NBP2-99507]

Western Blot: CD97 Antibody [NBP2-99507]

Western Blot: CD97 Antibody [NBP2-99507] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH/3T3 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: Raw264.7 Whole Cell Lysate. Lane D: Mouse spleen tissue lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa
Immunoprecipitation: CD97 Antibody [NBP2-99507]

Immunoprecipitation: CD97 Antibody [NBP2-99507]

Immunoprecipitation: CD97 Antibody [NBP2-99507] - CD97 was immunoprecipitated using: Lane A: 0.5 mg NIH/3T3 Whole Cell Lysate. 4 ul anti-CD97 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa

Applications for CD97 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD97

CD97, a CD55 Receptor, is a leukocyte antigen located on most activated leukocytes. CD97 is highly homologous to EMR2 with five EGF-like domains in the N-terminal extracellular domain, suggesting that it may be involved in cell signaling as well as in cell adhesion (Hamann et al., 1996). Two variants are produced by alternative splicing, where the shorter form lacks exons 5 and 6 and reduces the number of EGF-like domains from 5 to 3, compared to the full-length isoform. CD97 is associated with with the activated lesions of multiple sclerosis. CD97 expression is also implicated in the dedifferentiation of colon and thyroid tumors. CD97 is expressed abundantly in cells of hematopoietic origin and is markedly upregulated on activated T and B cells. ESTs have been isolated from a wide variety of tissue libraries.

Alternate Names

CD97

Gene Symbol

ADGRE5

Additional CD97 Products

Product Documents for CD97 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD97 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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