Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CDC25A Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 175 and 225 of human Cell Division Cycle 25A using the numbering given in entry NP_001780.2 (GeneID 993).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CDC25A Antibody - BSA Free (NB100-213) is a polyclonal antibody validated for use in WB and IP. Anti-CDC25A Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CDC25A Antibody - BSA Free

Western Blot: CDC25A Antibody [NB100-213]

Western Blot: CDC25A Antibody [NB100-213]

Western Blot: CDC25A Antibody [NB100-213] - Detection of human CDC25a by western blot. Samples: Nuclear extract (50 µg) from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: CDC25A Antibody [NB100-213]

Western Blot: CDC25A Antibody [NB100-213]

Western Blot: CDC25A Antibody [NB100-213] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CDC25a antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: CDC25A Antibody [NB100-213]

Western Blot: CDC25A Antibody [NB100-213]

Western Blot: CDC25A Antibody [NB100-213] - Detection of Human CDC25a by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibody: Affinity purified rabbit anti-CDC25a antibody NB100-213 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
CDC25A Antibody

Western Blot: Rabbit Polyclonal CDC25A Antibody [NB100-213] -

Western Blot: Rabbit Polyclonal CDC25A Antibody [NB100-213] - Whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. CDC25A Antibody (NB100-213) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.

Applications for CDC25A Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg of lysate

Reviewed Applications

Read 1 review rated 4 using NB100-213 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CDC25A

Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Long Name

Cell Division Cycle 25A

Alternate Names

CDC25A2, CDC25A2-CAG isoform, cell division cycle 25 homolog A (S. cerevisiae), cell division cycle 25 homolog A (S. pombe), cell division cycle 25A, Dual specificity phosphatase Cdc25A, EC 3.1.3.48, M-phase inducer phosphatase 1

Gene Symbol

CDC25A

Additional CDC25A Products

Product Documents for CDC25A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CDC25A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Phosphatases and Regulators

Citations for CDC25A Antibody - BSA Free

Customer Reviews for CDC25A Antibody - BSA Free (1)

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  • CDC25A Antibody
    Name: Nick Dordai
    Application: Western Blot
    Verified Customer | Posted 12/31/2023
    Western Blot: whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. CDC25A Antibody (NB100-213) was used for primary antibody: 1:1000, 4℃, overnight
    CDC25A Antibody - BSA Free NB100-213

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