CDC37 Antibody (10C3) - BSA Free

Novus Biologicals | Catalog # NBP3-26671

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10C3 expressed in HEK293

Format

BSA Free
View all CDC37 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human CDC37 [UniProt Q16543]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC37 Antibody (10C3) - BSA Free

CDC37 Antibody (10C3)

Western Blot: CDC37 Antibody (10C3) [NBP3-26671] -

Western Blot: CDC37 Antibody (10C3) [NBP3-26671] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate.
All lanes: CDC37 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 45 kDa
Observed band size: 50 kDa
CDC37 Antibody (10C3)

Flow Cytometry: CDC37 Antibody (10C3) [NBP3-26671] -

Flow Cytometry: CDC37 Antibody (10C3) [NBP3-26671] - Overlay histogram showing Hela cells stained with CDC37 Antibody (10C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for CDC37 Antibody (10C3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CDC37

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

Alternate Names

CDC37 (cell division cycle 37, S. cerevisiae, homolog), CDC37 cell division cycle 37 homolog, CDC37 cell division cycle 37 homolog (S. cerevisiae), CDC37A, cell division cycle 37 homolog (S. cerevisiae), Hsp90 chaperone protein kinase-targeting subunit, hsp90 co-chaperone Cdc37, P50CDC37

Gene Symbol

CDC37

Additional CDC37 Products

Product Documents for CDC37 Antibody (10C3) - BSA Free

Certificate of Analysis

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Product Specific Notices for CDC37 Antibody (10C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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