CDC37 [p Ser13] Antibody (5P8B2)

Novus Biologicals | Catalog # NBP3-33562

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 5P8B2
View all CDC37 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S13 of human CDC37 (Q16543).

Sequence:
EVSDD

Modification

p Ser 13

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CDC37 [p Ser13] Antibody (5P8B2)

CDC37 [p Ser13] Antibody (5P8B2)

Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] -

Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of lysates from C6 cells, using CDC37 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3min.
CDC37 [p Ser13] Antibody (5P8B2)

Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] -

Western Blot: CDC37 [p Ser13] Antibody (5P8B2) [NBP3-33562] - Western blot analysis of various lysates using CDC37 Rabbit mAb at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.

Applications for CDC37 [p Ser13] Antibody (5P8B2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDC37

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

Alternate Names

CDC37 (cell division cycle 37, S. cerevisiae, homolog), CDC37 cell division cycle 37 homolog, CDC37 cell division cycle 37 homolog (S. cerevisiae), CDC37A, cell division cycle 37 homolog (S. cerevisiae), Hsp90 chaperone protein kinase-targeting subunit, hsp90 co-chaperone Cdc37, P50CDC37

Gene Symbol

CDC37

Additional CDC37 Products

Product Documents for CDC37 [p Ser13] Antibody (5P8B2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CDC37 [p Ser13] Antibody (5P8B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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