CDC45L Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85723

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CDC45L Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELETAFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLIKQDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC45L Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CDC45L Antibody [NBP1-85723]

Immunocytochemistry/ Immunofluorescence: CDC45L Antibody [NBP1-85723]

Immunocytochemistry/Immunofluorescence: CDC45L Antibody [NBP1-85723] - Staining of human cell line A-431 shows localization to nucleoplasm & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723]

Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723]

Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723] - Staining in human testis and endometrium tissues using anti-CDC45 antibody. Corresponding CDC45 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723]

Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723]

Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723] - Staining of human endometrium shows low expression as expected.
Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723]

Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723]

Immunohistochemistry-Paraffin: CDC45L Antibody [NBP1-85723] - Staining of human testis shows high expression.
CDC45L Antibody - BSA Free Western Blot: CDC45L Antibody - BSA Free [NBP1-85723]

Western Blot: CDC45L Antibody - BSA Free [NBP1-85723]

Analysis in control (vector only transfected HEK293T lysate) and CDC45 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
CDC45L Antibody - BSA Free Western Blot: CDC45L Antibody - BSA Free [NBP1-85723]

Western Blot: CDC45L Antibody - BSA Free [NBP1-85723]

Analysis in human cell line K562.

Applications for CDC45L Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC45L

CDC45L is encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Alternate Names

CDC45 cell division cycle 45-like (S. cerevisiae), cell division cycle 45 homolog (S. cerevisiae), cell division cycle 45-like 2, human CDC45, S.cerevisiae, homolog)-like

Gene Symbol

CDC45

Additional CDC45L Products

Product Documents for CDC45L Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CDC45L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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