Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cdk7 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 300 and the C-terminus (residue 346) of human Cyclin-Dependent Kinase 7 using the numbering given in Swiss-Prot entry P50613 (GeneID 1022).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdk7 Antibody - BSA Free

Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581] - Detection of mouse and rat CDK7 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: Cdk7 Antibody [NB100-581]

Immunohistochemistry: Cdk7 Antibody [NB100-581]

Immunohistochemistry: Cdk7 Antibody [NB100-581] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 CDK7 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581] - RIPA lysate (5 and 25 mcg/lane in A; 5 mg in B) from HeLa cells. Affinity purified rabbit anti-CDK7 antibody used at 0.1 mcg/ml for WB and at 4 mcg/5 mg lysate for IP.
Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581] - Analysis using the Biotin conjugate of NB100-581. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes.
Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581] - Analysis of Biotin conjugate of NB100-581. Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, rabbit anti-CDK7 antibody used at 0.1 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time
Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581]

Western Blot: Cdk7 Antibody [NB100-581] - Detection of Human CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation: Cdk7 Antibody [NB100-581]

Immunoprecipitation: Cdk7 Antibody [NB100-581]

Immunoprecipitation: Cdk7 Antibody [NB100-581] - Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 (lot NB100-581-2) used for IP at 6 ug per reaction. CDK7 was also immunoprecipitated by a previous lot of this antibody NB100-581-1 For blotting immunoprecipitated CDK7, NB100-581 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Cdk7 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cdk7

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.

Long Name

Cyclin-dependent kinase 7

Alternate Names

CDKN7, MO15, p39 Mo15, STK1

Entrez Gene IDs

1022 (Human)

Gene Symbol

CDK7

UniProt

P50613 (Human)

Additional Cdk7 Products

Product Documents for Cdk7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cdk7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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