CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

Novus Biologicals | Catalog # NBP2-54626

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1H7-4B

Format

BSA Free
View all CEACAM-6/CD66c Primary Antibodies »

Product Specifications

Immunogen

Recombinant human CEACAM6/CD66c extracellular domain.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32031201).

Specificity

Clone 1H7-4B does not cross-react with human CEACAM1/3/4/5/7/8, rat CECAM1, or mouse CEACAM1/2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

Immunohistochemistry: CEACAM6/CD66c Antibody (1H7-4B) - BSA Free [NBP2-54626]

Immunohistochemistry: CEACAM6/CD66c Antibody (1H7-4B) - BSA Free [NBP2-54626]

Immunohistochemistry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Analysis in human lung: Using Anti-CEACAM6 [1H7-4B] (10 ug/ml) developed with DAB-staining procedure. Dark line indicated shows CEACAM6 expression.
Flow Cytometry: CEACAM6/CD66c Antibody (1H7-4B) - BSA Free [NBP2-54626]

Flow Cytometry: CEACAM6/CD66c Antibody (1H7-4B) - BSA Free [NBP2-54626]

Flow Cytometry: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM6 [1H7-4B] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
ELISA: CEACAM6/CD66c Antibody (1H7-4B) - BSA Free [NBP2-54626]

ELISA: CEACAM6/CD66c Antibody (1H7-4B) - BSA Free [NBP2-54626]

ELISA: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Solid phase was coated with 3 ug/ml Anti-CEA binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM6 [1H7-4B] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Immunoprecipitation: CEACAM6/CD66c Antibody (1H7-4B) - BSA Free [NBP2-54626]

Immunoprecipitation: CEACAM6/CD66c Antibody (1H7-4B) - BSA Free [NBP2-54626]

Immunoprecipitation: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM6 [1H7-4B]. Detection of the sample using 5 ug/ml pAb Anti-CEA followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.
CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

Western Blot: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] -

Western Blot: CEACAM-6/CD66c Antibody (1H7-4B) [NBP2-54626] - Detection using 10 μg/ml NBP2-54626 followed by HRP-coupled secondary antibody. Visualized by Chemiluminescence detection.

Applications for CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1 - 1000

Immunohistochemistry

1:100 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CEACAM-6/CD66c

CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. CEACAM 6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 6

Alternate Names

CD66c, CEACAM6, CEAL, NCA

Gene Symbol

CEACAM6

Additional CEACAM-6/CD66c Products

Product Documents for CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

Certificate of Analysis

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Product Specific Notices for CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CEACAM6/CD66c Antibody (1H7-4B) - BSA Free

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Protocols

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