Q: Do any of your recombinant proteins have DNA-binding activity? I need this as a control for some EMSAs I am doing to confirm my results obtained using nuclear extract.

A: NBC1-18419 is not expected to have DNA binding activity. The buffer is 20mM Tris-HCl pH7.5, 0.1M NaCl, 5mM beta-Mercaptoethanol and this will almost certainly denature the protein. It is intended to be used as a positive control. Unfortunately, we do not carry an active CEBP alpha protein at this time.

Recombinant Human CEBP alpha His Protein

Novus Biologicals | Catalog # NBC1-18419

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all CEBP alpha Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 270-358 of Human CEBP alpha

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGAG KAKKSVDKNS NEYRVRRERN NIAVRKSRDK AKQRNVETQQ KVLELTSDND RLRKRVEQLS RELDTLRGIF RQLPESSLVK AMGNCA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CEBP alpha His Protein

SDS-PAGE: Recombinant Human CEBP alpha His Protein [NBC1-18419]

SDS-Page: Recombinant Human CEBP alpha His Protein [NBC1-18419] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation, and Storage

NBC1-18419

Formulation	20 mM Tris-HCl buffer (pH 7.5) 0.1 M NaCl, 5mM beta-Mercaptoethanol.
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEBP alpha

CCAAT/enhancer binding protein(C/EBP) alpha is a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP family consist of several related proteins, C/EBP alpha, beta, gamma,delta, that form homodimers and that form heterodimers with each other. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein; a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. C/EBPs differ significantly in their physiological functions and in their downstream target genes. For example, mice lacking C/EBP alpha die shortly after birth due to severe hypoglycemia and the absence of glycogen storage in liver, whereas knockout of C/EBPbeta causes defects in female reproduction The bZip region of CEBP-alpha (residues 270-358)was produced in E.coli and purified by ion-exchange chromatography and FPLC gel-filtration chromatography.

Long Name

CCAAT/enhancer-Binding Protein alpha

Alternate Names

C/EBP-alpha, CEBPA

Gene Symbol

CEBPA

Additional CEBP alpha Products

Product Documents for Recombinant Human CEBP alpha His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human CEBP alpha His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Myeloid Leukemia

Basic Leucine Zipper (bZIP) Transcription Factors

Infectious Diseases

Interferons

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