CEBP Beta Antibody (3H9)

Name: CEBP Beta Antibody (3H9)
Brand: Novus Biologicals
SKU: NBP2-37567
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37567

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 3H9

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Product Specifications

Immunogen

Purified recombinant fragment of human CEBP Beta expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CEBP Beta Antibody (3H9)

Western Blot: CEBP Beta Antibody (3H9)BSA Free [NBP2-37567]

Western Blot: CEBP Beta Antibody (3H9) [NBP2-37567] - Western blot analysis using CEBPB mAb against human CEBPB (AA: 161-338) recombinant protein. (Expected MW is 44.5 kDa)

Flow Cytometry: CEBP Beta Antibody (3H9) - BSA Free [NBP2-37567]

Flow Cytometry: CEBP Beta Antibody (3H9) [NBP2-37567] - Flow cytometric analysis of MCF-7 cells using CEBPB mouse mAb (blue) and negative control (red).

ELISA: CEBP Beta Antibody (3H9) - BSA Free [NBP2-37567]

ELISA: CEBP Beta Antibody (3H9) [NBP2-37567] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Western Blot: CEBP Beta Antibody (3H9) [NBP2-37567] -

Silencing of KIFC1 and C/EBP beta reduces invasion and EMT in AR-TNBC cells. (A–D) Representative images (A) and bar graphs (B-D) of control (A, B–D), C/EBP beta KD (A, B), KIFC1 KD (A, C), and CW069-treated (A, D) and AR + TNBC and AR-TNBC cell lines. (E, F) Immunoblots showing expression levels of various EMT markers in KIFC1 KD (E) and C/EBP beta KD in AR + TNBC and AR-TNBC cells (F). HCC70, MFM223 (AR + TNBC); HCC1806, BT20 (AR-TNBC). Bars indicate mean +/- SEM. Unpaired two-tailed Student’s t-test with Welch’s correction was used to determine statistical significance. *P < 0.05, **P < 0.005, ****P < 0.00005, ns = non-significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40448099), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: CEBP Beta Antibody (3H9) [NBP2-37567] -

AR may repress C/EBP beta expression in breast cancer. (A–B, D) Correlation matrix of AR and C/EBP beta mRNA levels in TCGA (A), CCLE (B), and Emory Decatur (D) datasets. (C, F) Representative IHC images (C) and bar graphs (F) showing the expression of C/EBP beta in patients with AR + TNBC (n = 21) and AR-TNBC (n = 58) in the Emory Decatur cohort. (G, H) ChIP results showing AR binding to the C/EBP beta promoter (G). (E) Bar graphs showing mRNA levels of C/EBP beta in AR + TNBC and AR-TNBC cell lines. (I) Immunoblots showing C/EBP beta expression levels in AR + TNBC and AR-TNBC cell lines. (J, K) Schematic and bar graphs showing luciferase assay in AR + TNBC and AR-TNBC cells. HCC70, MFM223 (AR + TNBC); 3, 4: HCC1806, BT20 (AR-TNBC). Bars indicate mean +/- SEM. Unpaired two-tailed Student’s t-test with Welch’s correction was used to determine statistical significance. **P < 0.005, ***P < 0.0005, ns = non-significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40448099), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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Applications for CEBP Beta Antibody (3H9)

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEBP Beta

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth and differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP beta occurs predominantly as a heterodimer (3). C/EBP beta gamma readily heterodimerize with each other as well as with C/EBP alpha (4). Additionally, 3 isoforms of C/EBP beta can be detected (LAP, LAP,LIP)

Alternate Names

C/EBP beta, C/EBP-beta, CCAAT/enhancer binding protein (C/EBP), beta, CCAAT/enhancer-binding protein beta, CRP2, IL6DBP, interleukin 6-dependent DNA-binding protein, LAPTCF-5, Liver activator protein, liver-enriched transcriptional activator protein, MGC32080, NF-IL6, Nuclear factor NF-IL6, nuclear factor of interleukin 6, TCF5NFIL6, Transcription factor 5

Gene Symbol

CEBPB

Additional CEBP Beta Products

Product Documents for CEBP Beta Antibody (3H9)

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Certificate of Analysis

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Product Specific Notices for CEBP Beta Antibody (3H9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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