Cell Counting Kit-8

Key Product Details

Product Description

Cell Counting Kit-8 is a viability and proliferation assay test solution. It is a supplied as a ready-to-use 1-bottle solution (5 mL; 500 tests) and enables the colorimetric determination of the number of viable cells in cell proliferation and cytotoxicity assays. Cell Counting Kit-8 utilizes a highly water-soluble tetrazolium salt (WST-8), which is reduced by cellular dehydrogenases to an orange formazan dye. The color change can be detected in the 430 - 490 nm range and Cell Counting Kit-8 exhibits improved sensitivity compared with other tetrazolium salt-based assays such as MTT, XTT or MTS. Can also be used in the detection of superoxide.

For more information on how to use Cell Counting Kit-8, please see our protocol.

Scientific Data Images for Cell Counting Kit-8

Cell Number Determination.

HeLa cells 1x10⁴ cells/well in MEM were incubated overnight (37°C, 5% CO2) in clear 96-well plates. 10 μL Cell Counting Kit-8 or 10 μL MTT (Catalog # 5224) was added and incubated for a further 90 mins. The absorbance was measured at 450 nm or 570 nm, higher sensitivity was achieved via the Cell Counting Kit-8 assay.

Cytotoxicity Assay.

HeLa cells 1x10⁴ cells/well in MEM were incubated overnight (37°C, 5% CO2) in clear 96-well plates with staurosporine (Catalog # 1285). 10 μL Cell Counting Kit-8 or 10 μL MTT (Cat. No. 5224) was added and incubated for a further 90 mins. The absorbance was measured at 450 nm or 570 nm, higher sensitivity was achieved via the Cell Counting Kit-8 assay.

Product Specifications for Cell Counting Kit-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 7 publications citing the usage of this product.

• Tominaga A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Anal.Commun.
• Zhu Comparison of three analytical methods for superoxide produced by activated immune cells. J.Pharmacol.Toxicol.Methods 2020 PMID: 31678429
• Ishiyama A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta 1997 PMID: 18966866

Product Documents for Cell Counting Kit-8