Cell Counting Kit-8
Tocris Bioscience | Catalog # 7368
Cell viability and proliferation assay test solution
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Key Product Details
Description
Cell viability and proliferation assay test solution
Alternative Names
CCK-8,WST-8
Product Description
Cell Counting Kit-8 is a viability and proliferation assay test solution. It is a supplied as a ready-to-use 1-bottle solution (5 mL; 500 tests) and enables the colorimetric determination of the number of viable cells in cell proliferation and cytotoxicity assays. Cell Counting Kit-8 utilizes a highly water-soluble tetrazolium salt (WST-8), which is reduced by cellular dehydrogenases to an orange formazan dye. The color change can be detected in the 430 - 490 nm range and Cell Counting Kit-8 exhibits improved sensitivity compared with other tetrazolium salt-based assays such as MTT, XTT or MTS. Can also be used in the detection of superoxide.For more information on how to use Cell Counting Kit-8, please see our protocol.
Scientific Data Images for Cell Counting Kit-8
Cell Number Determination.
HeLa cells 1x104 cells/well in MEM were incubated overnight (37°C, 5% CO2) in clear 96-well plates. 10 μL Cell Counting Kit-8 or 10 μL MTT (Catalog # 5224) was added and incubated for a further 90 mins. The absorbance was measured at 450 nm or 570 nm, higher sensitivity was achieved via the Cell Counting Kit-8 assay.
Cytotoxicity Assay.
HeLa cells 1x104 cells/well in MEM were incubated overnight (37°C, 5% CO2) in clear 96-well plates with staurosporine (Catalog # 1285). 10 μL Cell Counting Kit-8 or 10 μL MTT (Cat. No. 5224) was added and incubated for a further 90 mins. The absorbance was measured at 450 nm or 570 nm, higher sensitivity was achieved via the Cell Counting Kit-8 assay.
Product Specifications for Cell Counting Kit-8
Storage
Store at +4°C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Calculators
Background References
References are publications that support the biological activity of the product. See our Citations tab to view 10 publications citing the usage of this product.
- Tominaga A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Anal.Commun.
- Zhu Comparison of three analytical methods for superoxide produced by activated immune cells. J.Pharmacol.Toxicol.Methods 2020 PMID: 31678429
- Ishiyama A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta 1997 PMID: 18966866
Product Documents for Cell Counting Kit-8
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cell Counting Kit-8
For research use only
Related Research Areas
Citations for Cell Counting Kit-8
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Species: RatAssay Type: In VitroVerified Customer | Posted 04/07/2026Two hours at 37°C it's enough to get good and replicable resultsTo address de difference in viability between two different cell lines
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