Catalog # Availability Size / Price Qty
Staurosporine | CAS No. 62996-74-1 | Broad Spectrum Protein Kinase Inhibitors
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Description: Non-selective protein kinase inhibitor
Alternative Names: AM-2282

Chemical Name: [9S-(9α,10β,11β,13α)]-2,3,10,11,12,13-Hexahydro-10-methoxy-9-methyl-11-(methylamino)-9,13-epoxy-1H,9H-diindolo[1,2,3-gh:3',2',1'-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one

Purity: ≥98%

Product Details
Citations (20)

Biological Activity

Staurosporine is a broad spectrum protein kinase inhibitor. Enzymes inhibited include protein kinase C, p60v-src tyrosine protein kinase, protein kinase A, and CaM kinase II (IC50 values are 3nM, 6 nM, 7 nM and 20 nM, respectively). Staurosporine reduces nuclear myosin heavy chain 9 phosphorylation which inhibits gastric cancer cell progression in transgenic mouse models. Staurosporine inhibits cell viability and promotes apoptosis in oral and pancreatic cancer cells. Staurosporine also enhances efficiency of lentiviral transduction of human hematopoietic stem and progenitor cells by 2-fold, induces dopaminergic axonal outgrowth in vitro and triggers mitophagy. Staurosporine has high affinity (Kd = 100 nM) for the yjdF aptamer. The riboswitch function of yjdF motif RNAs is activated by Staurosporine and leads to robust reporter gene expressions in B. subtilis.

Staurosporine synthesized to Ancillary Material Grade is also available.

Scientific Data

Cell Viability View Larger

Reduced viability of differentiated cardiomyocytes exposed to cardiotoxic small molecules. The viability of differentiated cardiomyocytes was assessed using a Resazurin metabolism assay. A) Untreated cells metabolize Resazurin (Catalog # AR002) to produce resorufin, a fluorescent molecule that can be measured using a fluorometric plate reader. Resorufin fluorescence accumulated in untreated cardiomyocytes. Cells treated with the cardiotoxic small molecules Staurosporine (blue; Catalog # 1285), a non-selective protein kinase inhibitor, or Doxazosin (tan; Catalog # 2964), an alpha 1 antagonist, did not metabolize Resazurin, as shown by lack of fluorescence accumulation, indicating a loss of viability upon treatment. B) Cell morphology of untreated, Staurosporine-treated, and Doxazosin-treated cardiomyocytes was assessed by brightfield microscopy.

Technical Data

Soluble to 50 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for Staurosporine

The citations listed below are publications that use Tocris products. Selected citations for Staurosporine include:

20 Citations: Showing 1 - 10

  1. High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cells.
    Authors: Hejna Et al.
    Sci Rep  2017;7:11943
  2. Matrine induces RIP3-dependent necroptosis in cholangiocarcinoma cells.
    Authors: Xu Et al.
    Cell Death Discov  2017;3:16096
  3. The microanatomic segregation of selection by apoptosis in the germinal center.
    Authors: Mayer Et al.
    Science  2017;358
  4. mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability.
    Authors: Valvezan Et al.
    Cancer Cell  2017;32:624
  5. Integrative genomic and functional analysis of human oral squamous cell carcinoma cell lines reveals synergistic effects of FAT1 and CASP8 inactivation.
    Authors: Hayes Et al.
    Cancer Lett  2016;383:106
  6. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS.
    Authors: Park Et al.
    Nat Immunol  2016;17:914
  7. Androgen-Sensitized Apoptosis of HPr-1AR Human Prostate Epithelial Cells.
    Authors: Chen Et al.
    Mol Pharmacol  2016;11:e0156145
  8. Identification of a DYRK1A Inhibitor that Induces Degradation of the Target Kinase using Co-chaperone CDC37 fused with Luciferase nanoKAZ.
    Authors: Sonamoto Et al.
    Eneuro  2015;5:12728
  9. Neurotensin Induces Presynaptic Depression of D2 DA Autoreceptor-Mediated Neurotransmission in Midbrain DArgic Neurons.
    Authors: Piccart Et al.
    J Neurosci  2015;35:11144
  10. FMRP regulates neurogenesis in vivo in Xenopus laevis tadpoles.
    Authors: Faulkner Et al.
    PLoS One  2015;2:e0055
  11. IF. gamma induces protective non-canonical signaling pathways in primary neurons.
    Authors: O'Donnell Et al.
    J Neurochem  2015;135:309
  12. Caffeine Modulates Vesicle Release and Recovery at Cerebellar Parallel Fibre Terminals, Independently of Calcium and Cyclic AMP Signalling.
    Authors: Dobson Et al.
    Eur J Neurosci  2015;10:e0125974
  13. Small molecule screen yields inhibitors of Pseudomonas homoserine lactone-induced host responses.
    Authors: Valentine Et al.
    Cell Microbiol  2014;16:1
  14. Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B.
    Authors: Barcomb Et al.
    J Biol Chem  2013;84:834
  15. Development of highly potent and selective diaminothiazole inhibitors of cyclin-dependent kinases.
    Authors: Schönbrunn Et al.
    J Med Chem  2013;56:3768
  16. Pharmacological inhibition of pleckstrin homology domain leucine-rich repeat protein phosphatase is neuroprotective: differential effects on astrocytes.
    Authors: Jackson Et al.
    J Pharmacol Exp Ther  2013;347:516
  17. Effect of activation of canonical Wnt signaling by the Wnt-3a protein on the susceptibility of PC12 cells to oxidative and apoptotic insults.
    Authors: Kawamoto Et al.
    PLoS One  2012;45:58
  18. Contractile effect of tachykinins on rabbit small intestine.
    Authors: Valero Et al.
    Acta Pharmacol Sin  2011;32:487
  19. Poly(ADP-ribose) polymerase (PARP)-1-independent apoptosis-inducing factor (AIF) release and cell death are induced by eleostearic acid and blocked by α-tocopherol and MEK inhibition.
    Authors: Kondo Et al.
    Braz J Med Biol Res  2010;285:13079
  20. N-myc down-regulated gene 1 modulates the response of term human trophoblasts to hypoxic injury.
    Authors: Chen Et al.
    J Biol Chem  2006;281:2764


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