Biological ActivityCELT-211 is a potent and selective fluorescent h5HT2B serotonin receptor ligand. It shows full selectivity for h5HT2B over h5HT2A, h5HT2C (Ki values is 56.3 nM for h5HT2B receptors in radioligand binding assay). Excitation and emission maxima (λ) are 589 and 616 nm, respectively. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.
Add 82 μL of assay buffer containing 1% of DMSO to obtain a 100 μM stock solution, and use 250 nM as a working concentration.
Optical Data for CELT-211
|Application||Time-resolved fluorescence energy transfer (TR-FRET)|
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