CG 858

Tocris Bioscience | Catalog # 7427

Selective BRAF-V600E protein Degrader (uSMITE™)
Tocris Bioscience

Key Product Details

Description

Selective BRAF-V600E protein Degrader (uSMITE™)
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Product Description

CG 858 is a selective BRAF-V600E protein Degrader (uSMITE™). CG 858 comprises the BRAF kinase inhibitor vemurafenib linked to the E3 ligase ligand Thalidomide (Cat. No. 0652). CG 858 dose-dependently degrades BRAF-V600E but not wild type BRAF, and reduces cell viability and colony formation in HT-29 and A375 cells (DC50 values are 124 nM and 500 nM, respectively). Exhibits 88% degradation at 500 nM. CG 858 also impairs melanoma cell growth.

CG 858 negative control (Cat. No. 7428) and B-Raf antibody validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF3424.

Licensing Information

Sold under license from Cullgen.

Product Specifications for CG 858

Molecular Weight

839.87

Formula

C42H39F2N7O8S

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

N-[[4-[3-[2,6-Difluoro-3-[(propylsulfonyl)amino]benzoyl]-1H-pyrrolo[2,3-b]pyridin-5-yl]phenyl]methyl]-5-[[2-(2,6-dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-5-yl]amino]pentanamide

CAS Number

2417296-82-1

PubChem ID

155551346

InChI Key

AAIPPJPUZKZIHC-UHFFFAOYSA-N

SMILES

CCCS(=O)(NC1=CC=C(C(C(C2=CNC3=NC=C(C4=CC=C(C=C4)CNC(CCCCNC5=CC=C6C(N(C(C6=C5)=O)C7CCC(NC7=O)=O)=O)=O)C=C23)=O)=C1F)F)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 83.99 100

Preparing Stock Solutions for CG 858

The following data is based on the product molecular weight 839.87.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.19 mL 5.95 mL 11.91 mL
5 mM 0.24 mL 1.19 mL 2.38 mL
10 mM 0.12 mL 0.60 mL 1.19 mL
50 mM 0.02 mL 0.12 mL 0.24 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for CG 858

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CG 858

For research use only

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