Human B-Raf Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human B-Raf in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human B-Raf
    Met1-His766
    Accession # P15056
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human B-Raf by Western Blot. Western blot shows lysates of T47D human breast cancer cell line, MDA‑MB‑453 human breast cancer cell line, Daudi human Burkitt's lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line and U266 human myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B-Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for B-Raf at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human B‑Raf by Simple WesternTM. Simple Western lane view shows lysates of T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for B‑Raf at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human B‑Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Detection of Human B‑Raf by Simple WesternTM. Simple Western lane view shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for B‑Raf at approximately 95 kDa (as indicated) using 10 µg/mL of Goat Anti-Human B‑Raf Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3424) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B-Raf

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: Raf-1 (C‑Raf); A-Raf; and B-Raf, found at high levels in cerebrum and testes. Mice with a targeted disruption of the B-Raf gene die of vascular defects during mid-gestation. B-Raf mutations have been found in two-thirds of malignant melanomas, with the single substitution V599E in the kinase domain the most frequent occurrence. B-Raf activation in melanomas results in BIM phosphorylation and inhibition of apoptosis.

  • Long Name:
    v-Raf Murine Sarcoma Viral Oncogene Homolog B1
  • Entrez Gene IDs:
    673 (Human); 114486 (Rat)
  • Alternate Names:
    94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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