Key Product Details

Species Reactivity

Chicken

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant chicken RGM-A
Trp30-Asn404 (Asn404Asp)
Accession # Q8JG54

Specificity

Detects chicken RGM in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Chicken RGM‑A Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Chicken Repulsive Guidance Molecule A/RGM-A

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RGM-A

Chicken RGM is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)-anchored axon guidance proteins. It is a disulfide-linked dimer of the N-terminal RGD-containing segment (aa 30‑149) and the C-terminal segment (aa 150‑432). RGM binds with high affinity to neogenin. Chicken RGM (aa 30‑401) shares 88% and 87% aa sequence identity with its human and mouse homologs, respectively.

Long Name

Repulsive Guidance Molecule A

Alternate Names

RGMA

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Gene Symbol

RGMA

UniProt

Additional RGM-A Products

Product Documents for Chicken RGM‑A Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Chicken RGM‑A Biotinylated Antibody

For research use only

Related Research Areas

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FAQs

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