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Chicken RGM-A Alexa Fluor® 594-conjugated Antibody

Catalog #: AF2010T Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2010T-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Chicken RGM-A Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Chicken
Specificity
Detects chicken RGM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human RGM and recombinant mouse RGM.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant chicken RGM-A
Trp30-Asn404 (Asn404Asp)
Accession # Q8JG54
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RGM-A

Chicken RGM is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)-anchored axon guidance proteins. It is a disulfide-linked dimer of the N-terminal RGD-containing segment (aa 30‑149) and the C-terminal segment (aa 150‑432). RGM-A binds with high affinity to neogenin. Chicken RGM-A (aa 30‑401) shares 88% and 87% aa sequence identity with its human and mouse homologs, respectively.

Long Name
Repulsive Guidance Molecule A
Entrez Gene IDs
56963 (Human); 244058 (Mouse)
Alternate Names
RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A

Product Datasheets

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Product Datasheet
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